Hacia una nueva filogenia de Tulasnella mediante la combinación de descriptores moleculares

Abstract

The high genetic variability that occurs in mycorrhizal fungi of the genus Tulasnella in the ITS region of the gene nrDNA difficult to generate statistically reliable phylogenies by methods that depend on the alignment of ITS sequences. As a result of this variability phylogenies it is often ambiguous, that do not allow adequate correlation of molecular data (ITS sequence), and morphological obtained for pure or isolated basidiomata of Tulasnella spp. From this, in the present study new methodologies are proposed to increase the reliability of phylogenies obtained for Tulasnella using distance matrices. To this end ITS sequences belonging to 383 species of Tulasnella were used. Phylogenies were obtained from three strategies: 1) single multiple alignment, not only dependent descriptors structural alignment and 3) a combination of 1) and 2) using the statistical procedure known as DISTATIS. Our results allowed us to conclude that the best phylogenies are obtained from alignment and DISTATIS. The new phylogenies obtained open the possibility of reconciling molecular information with morphological isolates Tulasnella