Caracterización molecular y conservación de hongos del suelo asociados con la respuesta a estrés por nitrógeno

Abstract

Biological nitrogen fixation is a process that provides a 65% annual N fixation ecosystem; executed by fungal microorganisms capable of reducing air form of N in the form assimilable. There are several human activities that have favored increased N addiction, which has led to duplication of N which is built per year in different soil biological cycles. This study aims to characterize molecularly and cryopreserved fungi associated with the stress response by N. Selecting soils subjected to high stress by addition of N with soil control, which cultivable fungi was isolated to be characterized by molecular techniques. Which resulted in 43 strains characterized by basic morphology. Phylogeny indicated the presence of the phylum Ascomycota two clades. Eurotiales (cf. Citrinum Penicillium, Aspergillus and Aspergillus sp cf. Nigger). Hypocreales clade (Metarrhzium sp. Clonostachys Tolypocladium sp and sp) and gender zygomycota phylum Morlierella sp. The percentage of recovery of cryopreserved strains was 98%.