Caracterización morfo-molecular de hongos anamorfos del cepario UTPL y evaluación de su potencial antibacteriano

Abstract

Fungi have an enormous diversity, little known. They survive in different environments so they are important synthesizers of a high diversity of chemical compounds. In this context, this study aims to characterize morphologically and molecularly 20 strains of anamorphic fungi and to evaluate their antimicrobial potential through antagonistic tests with bacteria of clinical interest. The morphological identification was carried out by the description and comparison of microscopic structures. Molecularly they were characterized by amplifying the region ITS-5.8S and LSU of rDNA. The antimicrobial potential was evaluated through the agar diffusion method and considered positive when they presented inhibition halos. The MIC was determined from the extracts by the broth microdilution method. Integrating the morphological and molecular data, 11 strains corresponded to species within five genera of ascomycetes. Two species Trichoderma sp.1 and Trichoderma sp.2 generated halos of inhibition against Escherichia coli, Klebsiella pneumoniae, Pseudomona aeruginosa, Proteus vulgaris, Salmonella typhimurium and Staphylococcus aureus. The Minimum Inhibitory Concentration values showed that the extracts of these two species were inefficient against the evaluated bacteria