Prevalencia de las especies termotolerantes de Campylobacter (C. jejuni subsp. jejuni, C. coli, C. lari y C. upsaliensis) en niños con y sin diarrea del Hospital del Día, durante el periodo septiembre - diciembre 2014.

Abstract

Campylobacter is a zoonotic bacteria, causes campylobacteriosis, provoking approximately 5-14% of diarrhea worldwide being most affected children, elderly and individuals immunocompromised. Due to the clinical and socioeconomic importance of this bacteria was determined prevalence of thermotolerant species of the Campylobacter (C. jejuni, C. coli, C. lari and C. upsaliensis) in children with and without diarrhea of the Day Hospital during the period September to December 2014. Were collected 79 Fecal samples, being obtained 7 positive cultures; being C. jejuni and C. coli, the isolated species, corresponding to a frequency of 9%. Species identification was made considering the metabolic characteristics of Campylobacter, against hydrolysis of hippurate. The Bacterial susceptibility was determined by the diffusion method in Mueller Hinton agar with 5% blood as recommended CA-SFM / EUCAST, Getting resistance to: ciprofloxacin tetracycline 71% and ampicillin 14%. Subsequently is conducted a MULTIPLEX-PCR in order to complement the results obtained by conventional biochemistry. The results show that the prevalence of Campylobacter in this health institution corresponds to 9%.