Establecimiento de procedimientos para el aislamiento, cultivo y fermentación de hongos basidiomicetos colectados en bosque húmedo tropical

Abstract

Tropical rain forests are areas of high biodiversity in Ecuador located in the South Zone 7 region, rich in biodiversity of flora, fauna and mycobiota; the latter representing fungi distinguished by their function, mycelium and variety. Basidiomycetes macrofungi collection was performed by mycological expeditions for five months in 2014, in wooded areas randomly selected Saraguro and Zamora. Of the 52 specimens were obtained primary cultures, insulation and pure cultures, with a successful morphological and molecular identification. Pycnoporus sanguineus and Fuscoporia sp. species were studied using different solids and liquids cultures, evaluating their radial growth and extracts obtained. The analysis indicate that the YNPD medium gives abundant growth while SDA medium is optimal to favor fermentation. The extracts highlighted in quantity were obtained from spent culture, therefore, different compounds obtained from secondary metabolism by extraction with chloroform were observed using TLC; and in MIC present biological activity of 500 μg/ml for Trichophyton mentagrophytes and Trichophyton rubrum, and 1000 μg/ml for Enterococcus faecalis.