Estudio molecular de resistencia bacteriana a antibióticos b-lactámicos en hospitales de Loja

Abstract

Abstract: Gram negative bacteria use the enzyme modification mechanism to survive the activity of beta-lactam antibiotics, through production of beta-lactamase plasmid-encoded that transform into a derivative antibiotic without bactericidal activity, due to the inappropriate use of these drugs. This research project focused on determining qualitative and quantitative gene variants that encode different types of beta-lactamases BLEES and AmpC present in resistant bacterial strains from hospitals Manuel Ygnacio Monteros and Hospital Day Ecuadorian Social Security Institute IESS of the city of Loja, by applying techniques such as multiplex PCR, sequencing and sequence comparison in the online program BLAST. Based on the results obtained, we determine a high prevalence of genes type BLEE whose gene group TEM was predominant, while within the low frequency of genes type AmpC became to prevail the group gene DHA, besides we identified the frequencies of different variants for each gene group, reaching to ratify the spread of these resistance genes in pathogenic bacteria gram negative.