Aislamiento e Identificación de microorganismos de sedimentos y aguas de piscinas camaroneras y su posterior inactivación mediante procesos de oxidación avanzada

Abstract

Abstract: In the present study, the isolation of four bacteria obtained from water samples from shrimp ponds in the Huaquillas canton, province of El Oro was carried out and, through molecular analysis of the 16S rRNA region, they were identified as Exiguobacterium sp., Bacillus sp., Klebsiella pneumoniae, and Vibrio fluvialis,. The inactivation of the K. pneumoniae bacteria was assessed using advanced oxidation treatments with UV: (UV, UV/H2O2, UV/H2O2/Fe) at different concentrations of hydrogen peroxide (10, 30 and 60 ppm) and irradiation times. (0, 16, 26, 36, 46, 56 seconds) at neutral pH. It was determined that the addition of 10 ppm of hydrogen peroxide (UV/H2O2) and 10 ppm of hydrogen peroxide with 2 ppm of iron (UV/H2O2/Fe) turned out to be the most effective treatments, allowing greater inactivation with less irradiation time. The remaining treatments were found to be less effective with longer inactivation times and similar bacterial clearance rates. Advanced oxidation processes in relation to other treatments turn out to be an efficient technique for disinfecting shrimp cultures.