Actividad anti-genotóxica mediante ensayo cometa de la horchata expendida en los mercados de Loja

Abstract

Traditional medicine has been of great interest worldwide because it involves the use of medicinal plants for the treatment of diseases. In Ecuador, the horchata is a drink made with everyday plants that have therapeutic properties, however, it is known that many of these plants can be toxic to health. In this paper we evaluate the genotoxic activity and anti-genotoxic 9 different types of horchata on the cell line of Chinese Hamster Ovary (CHO-K1) by comet assay and production and inhibition of reactive oxygen species by fluorimetry. The results indicate that horchatas exert genotoxic effects in the DNA of cells and ROS production, however, when combined with an inducer of genotoxic damage and ROS, decreases both genotoxicity and ROS production, which we It confirms that this traditional drink has anti-genotoxic and antioxidant activity. We compare our results with the composition of horchata and found that the presence or absence of some plants could be influencing for this effect to occur.