Aislamiento y caracterización de metabolitos secundarios a partir de Erythrina ulei con posible actividad antiparasitaria

Abstract

Erythrina ulei species were collected from the rural parish of Cumbaratza, Zamora Chinchipe province, Ecuador. Nine extracts were obtained using various solvents as hexane, ethyl acetate and methanol. For the flowers, methanol extract had the highest extract efficiency with 10.94%. The flower and leaf ethyl acetate extracts were analyzed by open column chromatography. A further alkaloid extraction procedure was performed on the leaf methanol extract. From the ethyl acetate flower extract we obtained two distinct mixtures; mixture 1: tricosane, tetracosane, pentacosane, hexacosane, heptacosane, octacosane, nonacosane, eicosane; mixture 2 could probably be: bikaverin, stigmastan 3,5,22 trien and stigmastan 3, 5 dien. The phytol acetate compound of the leaf ethyl acetate extract was identified. From the leaf methanol extract we obtained 236 and 226 mg of alkaloids extract at pH7 and pH 11 respectively. For compound identification we used gas chromatography coupled to mass spectroscopy (GC/MS), 1H and 13C RMN, retention index, melting point and retention factor.