Extracción, aislamiento y caracterización de metabolitos secundarios del extracto en diclorometano de la especie Loricaria thuyoides (Lam.) Sch. Bip

Abstract

Abstract: From the leaves of the medicinal plant Loricaria thuyoides known as Trencillo de cerro , collected in the Saraguro canton, the extraction, isolation and characterization of secondary metabolites was carried out using NMR and GC-MS spectroscopic techniques. Identifying two coumarins known as scopoletin and 5-hydroxy-obliquine. Additionally, the isolation of two compounds of terpenic origin (named KE43-27 and KE194-73) and a mixture of fatty acids was carried out. The inhibitory activity of the enzymes acetylcholinesterase and butyrylcholinesterase of the isolates was evaluated. The results showed that the compound KE43-27 showed a significant inhibitory activity against the enzymes acetylcholinesterase and butyrylcholinesterase with concentrations of 3.06 and 3.42 mU/ml respectively. While the compound 5-hydroxy-obliquin only showed inhibitory activity to the enzyme butyrylcholinesterase (18.4 mU/ml), on the other hand, the compound KE194-73 did not show inhibitory activity for the enzymes tested.