Caracterización morfo-molecular de hongos anamorfos del cepario UTPL y evaluación de su potencial antibacteriano

Abstract

Abstract: Fungi have an enormous but little known diversity. They survive in different environments so they are important synthesizers of a high diversity of chemical compounds. In this context, this study aims to characterize morphologically and molecularly 20 strains of anamorphic fungi and evaluate their antimicrobial potential through antagonistic tests including bacteria with clinical interest. The morphological identification was carried out by the description and comparison of microscopic structures. Molecularly they were characterized by amplifying the region ITS-5.8S and partial LSU of rDNA. The antimicrobial potential was evaluated through the agar diffusion method and considered positive in presence of inhibition halos. The Minimum Inhibitory Concentration MIC was determined from the extracts by the broth microdilution method. Integrating the morphological and molecular data, 11 strains correspond to different species within five genera of ascomycetes. Two species Trichoderma sp.1 and T. sp.2 generate inhibition halos against Escherichia coli, Klebsiella pneumoniae, Pseudomona aeruginosa, Proteus vulgaris, Salmonella typhimurium and Staphylococcus aureus. The MIC values shows that the extracts for the two species were inefficient against the evaluated bacteria.