Evaluación de velocidad de crecimiento y esporulación de cuatro cepas de hongos micorrízicos de orquídeas en dos medios de cultivo

Abstract

The genus Tulasnella, an important mycobionte of orchids, presents difficulties of isolations and low rates of growth in vitro. Few studies have achieved their isolation and sporulation from sexual states. According to this problem, taxonomic and ecological studies have constant limitations. Our study aims to evaluate different sources of cellulose, which allow to increase the growth of different strains of Tulasnella spp. Isolated from epiphytic orchids. The strains were replicated in six treatments of Alnus, wheat and canary seed in medium (Bacto / MEA) with their respective controls (Bacto / MEA). Statistically the data of the mycelial diameter of the strains were analyzed with ANOVA and Tukey's test. Sexual or asexual sporulation was evaluated microscopically after subjecting the strains to temperature stress (4 °, 22 ° and 27 ° C) for four months. The ANOVA with (p-value ≤ 0.001) and the Tukey test established that the treatments (wood / Bacto, wheat Bacto / MEA, canary seed / MEA) positively influence the growth of Tulasnella spp. In addition, the treatments (wood Bacto / MEA and wheat / Bacto) favored the formation of asexual spores in two of the evaluated strains HUTPL (CF) 243 and 36 of Tulasnella spp.