Please use this identifier to cite or link to this item: http://dspace.utpl.edu.ec/jspui/handle/123456789/18949
Title: Metabolic profiling of alpine and ecuadorian lichens
Authors: Mittermeier, V.
Eisenreich, W.
Volk, L.
Schmitt, N.
Beck, A.
Suarez Chacon, J.
Keywords: Chemotaxonomy
Metabolomics
Principle component analysis
Stereocaulon
Sticta
Sticticin
metadata.dc.date.available: 2017-06-16T22:02:40Z
Issue Date: 1-Oct-2015
Publisher: Molecules
Abstract: Non-targeted 1H-NMR methods were used to determine metabolite profiles from crude extracts of Alpine and Ecuadorian lichens collected from their natural habitats. In control experiments, the robustness of metabolite detection and quantification was estimated using replicate measurements of Stereocaulon alpinum extracts. The deviations in the overall metabolite fingerprints were low when analyzing S. alpinum collections from different locations or during different annual and seasonal periods. In contrast, metabolite profiles observed from extracts of different Alpine and Ecuadorian lichens clearly revealed genus- and species-specific profiles. The discriminating functions determining cluster formation in principle component analysis (PCA) were due to differences in the amounts of genus-specific compounds such as sticticin from the Sticta species, but also in the amounts of ubiquitous metabolites, such as sugar alcohols or trehalose. However, varying concentrations of these metabolites from the same lichen species e.g., due to different environmental conditions appeared of minor relevance for the overall cluster formation in PCA. The metabolic clusters matched phylogenetic analyses using nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequences of lichen mycobionts, as exemplified for the genus Sticta. It can be concluded that NMR-based non-targeted metabolic profiling is a useful tool in the chemo-taxonomy of lichens. The same approach could also facilitate the discovery of novel lichen metabolites on a rapid and systematical basis. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
metadata.dc.identifier.other: http://10.3390/molecules201018047
URI: http://dspace.utpl.edu.ec/handle/123456789/18949
ISBN: 14203049
Other Identifiers: http://10.3390/molecules201018047
Other Identifiers: http://10.3390/molecules201018047
metadata.dc.language: Inglés
metadata.dc.type: Article
Appears in Collections:Artículos de revistas Científicas



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