Aislamiento biodirigido (in vitro) de compuestos antioxidantes y antihiperglucemiantes a partir de Ilex guayusa

Abstract

Abstract: In this research the anti-hiperglucemiant and antioxidant activity was evaluated from the extracts: hexanic, ethyl and methanolic acetate; of the vegetal species llex guayusa which is traditionally used in the treatment of diabetes mellitus, moreover the division of the active extract was done. We used some methods which allow to determine the antioxidant and antiglucemiant capacity of the extracts, such as the inhibition of the enzyme a-glucosidase for antiglucemiants and ẞ-Clams, DPPH, Folin ciocalteu to antioxidants using different concentrations from the extracts and their respective positive and negative controls. In all the applied tests we obtained that the methanolic extract presents a great activity in antidiabetics as in antioxidants. The division of the methanolic extract gives as a result a pure mixture belong to the triterpenos.