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Title: | Aislamiento de bacterias con potencial biodegradador de residuos plásticos sintéticos |
Authors: | Herrera Vargas, Paulo Ignacio Llanes Sarango, Karina del Carmen |
Keywords: | Ecuador. Tesis digital. |
Issue Date: | 2022 |
Citation: | Llanes Sarango, K. D. C. Herrera Vargas, P. I. (2022) Aislamiento de bacterias con potencial biodegradador de residuos plásticos sintéticos [Tesis de Grado, Universidad Técnica Particular de Loja]. Repositorio Institucional. https://dspace.utpl.edu.ec/handle/123456789/30808 |
Abstract: | Abstract:Plastic is a material that offers numerous uses and applications in any area of human life. This has caused an environmental problem due to plastic waste, threatening the health of living beings. The objective of this study is to isolate and identify bacteria with plastic degrading capacity from samples of commercial plastic bottles made of polyethylene terephthalate (PET) and low-density polyethylene (LDPE) shopping bags. For this purpose, 41 samples were collected from two contaminated sites in the province of Loja, Ecuador. A total of 216 bacterial strains were isolated on nutrient agar and TSA. Subsequently, we evaluated their degradation potential in liquid Mineral Medium (MM) using PET and LDPE sheets. Of which 33 bacterial strains showed such degradative potential. Eight bacteria were identified, being Moraxella sp. with the highest percentage of LDPE degradation (24.24%) and Enterococcus sp. the isolate with the highest percentage of PET degradation (5.31%). The bacterial strains obtained will be useful for evaluating enzymes involved in the decomposition processes of contaminating plastics in the next phase of the project. |
Description: | Resumen:El plástico es un material que ofrece numerosos usos y aplicaciones en cualquier ámbito de la vida del ser humano. Esto ha ocasionado un problema ambiental por residuos plásticos, amenazando la salud de los seres vivos. El objetivo de este estudio es aislar e identificar bacterias con capacidad degradadora de plásticos de muestras de botellas plásticas de uso comercial elaboradas de tereftalato de polietileno (PET) y bolsas de compra de polietileno de baja densidad (LDPE). Para ello, se colectaron 41 muestras en dos sitios contaminados de la provincia de Loja, Ecuador. Se aisló 216 cepas bacterianas en agar nutritivo y TSA. Posteriormente evaluamos su potencial degradador en Medio Mineral líquido (MM) empleando láminas PET y LDPE. De las cuales 33 cepas bacterianas mostraron dicho potencial degradador. Se identificaron 8 bacterias, siendo Moraxella sp., con mayor porcentaje de degradación de LDPE (24,24%) y Enterococos sp., el aislado con mayor porcentaje de degradación de PET (5,31%). Las cepas bacterianas obtenidas serán de utilidad para, en una siguiente fase del proyecto, evaluar enzimas involucradas en procesos de descomposición de plásticos contaminantes. |
URI: | https://bibliotecautpl.utpl.edu.ec/cgi-bin/abnetclwo?ACC=DOSEARCH&xsqf99=130087.TITN. |
Appears in Collections: | Titulación de Bioquimica y Farmacia |
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