Producción de biohidrógeno a partir de un licor hidrolizado de Jacinto de agua con Clostridium sp degenerado

Abstract

Abstract:This research project has as principal objective to determine the biohydrogen yield obtained after of the anaerobic fermentation process using a degenerate strain of Clostridium acetobutylicum ATCC824. In order to this objective, the Eicchornia crassipes specie has been used as a source of carbon and energy, which is a vegetal specie featured to be a lignocellulosic material and also considered as a plague due to its accelerate growth in lakes and rivers, producing a negative effect in the biota, for this reason it's necessary to take advantage of this residue. In this project, a hydrolyzed liquor was obtained from the use of the cellulase from Trichodermea reesei ATCC26921 enzyme which produced a glucose yield of 63% percent, the kinetics study showed a Vmax of 0,387 g·L -1h -1 and a Km of 38,87 g·L -1 . The results of the biohydrogen production, using Clostridium acetobutylicum ATCC824 degenerate, were evaluated taking as a base the proportion of liquor hydrolyzed Eicchornia crassipes 50% percent as carbon source which allows the microbial growth, the volume of biohydrogen was of 3,65 L, the volumetric productivity after the anaerobic fermentation of the 0,61 g·L -1h -1 and according to the theoretical and experimental analysis the yield was estimated performance is 3,95 moles of hydrogen per mole of glucose consumed.