Actividad de glutatión peroxidasa (GPX) y superóxido dismutasa (SOD) en respuesta a agentes tóxicos

Abstract

Abstract: Exposure to toxic agents can induce oxidative stress in cells, as well as cause biochemical alterations due to the accumulation of free radicals. However, cells have antioxidant defense mechanisms such as superoxide dismutase and glutathione peroxidase to eliminate reactive species and maintain cellular homeostasis. Therefore, the present study aims to evaluate the activity of glutathione peroxidase (Gpx) and superoxide dismutase (SOD) in response to pesticide exposure. The determination of enzyme activity was evaluated in the CHO-K1 cell line treated with pesticides frequently used in the province of Loja, at 4-, 24- and 48-hour exposure periods. The results of the present study showed no significant differences in SOD activity with respect to the untreated control. However, Gpx activity showed an increase in response to exposure, which could be explained as the activation of cellular mechanisms of adaptability to the surrounding environment for cell survival.