Análisis químico y enantioselectivo de la fracción volátil de Gynoxys buxifolia de la provincia de Loja

Abstract

Abstract: The vegetable matter Gynoxys buxifolia (Asteraceae), the volatile fraction was obtained by steam stripping. The chemical analysis GC-MS (qualitative) and GC-FID (quantitative) was carried out in polar and non-polar columns, identifying 72 compounds both in the essential oil and in the hydrosol. The essential oil identified (87.6% and 83.9%) consisted mainly of oxygenated sesquiterpenes (61.7% and 57.9%), where two of these were identified and isolated by NMR, Furanoeremophilane (C₁₅H₂₂O) (31.3% and 28.3%) and Bakkenolide A (C₁₅H₂₂O₂) (17.6% and 16.3%). The aqueous phase (hydrolate) was also exposed to a chemical analysis identified (40.7 mg/100ml and 43.6 mg/100ml) showing p-vinyl-Guaiacol with (25.4 mg/100mL and 29.9 mg/100mL) as the major compound. The essential oil was then analyzed to determine the enantiomeric distribution of its monoterpene constituents, yielding the following enantiomeric excesses on a β-cyclodextrin-based enantioselective column: (1R,5R)-(-)-α-pinene, (1S,5S)-(-)-β-pinene (100%), (R)-(+)-Sabinene (15%), (S)-(-)-Sabinene (85%), (S)-(+)-α-Phellandrene (100%), (S)-(+)-β-Phellandrene (100%), (S)-(-)-Terpinen-4-ol (100%)