Aislamiento e identificación molecular de bacterias de la rizósfera del banano como potenciales biocontroladores de Fusarium

Abstract

Abstract: Rhizobacteria are a large group of bacteria that inhabit the rhizosphere of plants promoting their growth through different mechanisms, such as: nitrogen fixation, mineral uptake and biocontrols of different phytopathogens such as Fusarium oxysporum f. sp. Its study and use promote sustainable agriculture and higher crop yields. The rhizosphere samples used in the study came from a plantation of banana on a small scale and without the application of agrochemicals located in the canton Alamor, province of Loja. The isolation of rhizobacteria in pure cultures was carried out in the middle Agar starch M-protein agar. A total of sixteen cultures were obtained pure. Subsequently, the morphological characterization (macroscopic and microscopic) and molecular of the same. The results indicate the presence of several isolated mainly within the genus Bacillus and Pseudomonas. Although I do not know performed antagonism tests against Fusarium oxysporum f. sp. is known to through the consulted bibliography that many of them could have an effect for control the growth of this pathogen, so they could be used in trials to reduce the use of chemical products, which cause damage to the Human health.