Aislamiento e identificación de metabolitos secundarios del extracto en metanol de las hojas de Siparuna muricata (Ruiz & Pav.) A. DC

Abstract

Abstract:Siparuna muricata is an aromatic native plant with a characteristic citrus smell, which is It is found in the mountains in the provinces of Loja, Zamora Chinchipe, Napo, Bolívar, Azuay, Chimborazo, Cañar, Imbabura and Tungurahua, the genus of this species has demonstrated a wealth of secondary metabolites that stand out as alkaloids, flavonoids, tannins, coumarins and cardiac glycosides. The present research work seeks the isolation and identification of secondary metabolites from the methanol extract of the leaves of Siparuna muricata (Ruiz & Pav.) A. DC using chromatographic techniques such as: Thin Layer Chromatography (TLC) and Column Chromatography (CC), as well as techniques spectroscopic as: NMR, HPLC-MS and IR. The plant sample was collected in the sector of the Villonaco hill in the province of Loja, 500 gr of the plant were taken, macerated and concentrated in a BUCHI R-210 rotary evaporator, obtaining a yield of 19.07%. By Therefore, the genus Siparuna has been shown to have a wealth of secondary metabolites. such as sesquiterpenes, flavonoids and isoquinoline alkaloids to which attaches great biological importance.