Aislamiento y caracterización de metabolitos secundarios de fracciones de polaridad media de Tragia volubilis L

Abstract

Abstract:Tragia volubilis L commonly known as nettle, belongs to the Euphorbiaceae family and has a wide distribution of 6300 species and 245 genera, it is commonly used in traditional medicine as an anticancer and antitumor agent. Our work group in previous studies evaluated the antioxidant capacity of the methanolic and aqueous extracts and of several partitions of the aqueous extract. The highest index of antioxidant activity was shown by the ethyl acetate (H2O/AcOEt) and n-butanol (H2O) partitions. /BuOH) from the aqueous extract; In the present work, the partition of ethyl acetate (H2O/AcOEt) was used to isolate secondary metabolites present, using different polarities and proportions of solvents, which found an exact one in methanol dichloromethane 1:1, using reversed phase preparative chromatography and using 1:1 dichloromethane-methanol reagent grade solvents, managing to isolate by preparative chromatography and characterize by NMR a glycosylated flavonoid: quercitrin (quercetin 3-O rhamnoside). Tests were made with different polarities and proportions of solvents for the butanol (H2O/BuOH) partitioning of the aqueous extract, but it was not possible to find an adequate polarity to be able to perform preparative chromatography and try to isolate the secondary metabolites present.