Aislamiento y evaluación de rizobacterias de Calendula officinalis L., para el control in vitro del nemátodo Meloydogine incognita Kofoid y White (1919), del tomate de riñón (Solanum lycopersicum L.)

Abstract

Abstract:Rhizobacteria inhabit the rhizosphere of plants and can be used as Biological controllers against various pests. Calendula officinalis L. is a potential study plant, therefore, the objective of this research was to isolate rhizobacteria from Calendula officinalis L. and evaluate its capacity as a bio-controller of the nematode Meloydogine incognita from the culture of Solanum lycopersicum L., under in vitro conditions. HE used a completely randomized design with six treatments and five repetitions. HE isolated and morphologically characterized 12 colonies of rhizobacteria, finding that the strains 2, 3 and 9 have morphological characteristics similar to the genus Bacillus. And then In vitro dual confrontations were performed, 0.35: 1.5 ml (nematodes: bacterial filtrates). Resulting that the treatments T2, T3, T4 and T5 have a significant effect on mortality of M. incognita J2 at 24 and 48 hours. Especially T4 that had a greater effectiveness 70% after 48 hours of exposure. Therefore, it is concluded that the filtered rhizobacterials had nematicidal effects on M. incognita (J2) and its use is recommended. use as potential Bio-controllers.