Composición química del extracto alcohólico de Gynoxys cuicochensis Cuatrec.

Abstract

Abstract: Gynoxys cuicochensis Cuatrec, an Asteraceae that inhabits the páramo of Fierro Urco de la province of Loja, is a striking species for its yellow inflorescence. To know your secondary metabolites and possible applications, a phytochemical characterization was carried out of the ethanolic extract of the plant, obtained by static maceration for a period of 3 days for the first filtration, and one day for the second and third filtration. The chlorophyll was separated using a solid phase of Diaon Hp 20 resin, the same one that was packed in separatory funnels, and a liquid phase of EtOH:H2O in concentration gradient from 6:4 to 9:1 for elution. The dechlorophylled extract was lyophilized and subsequently fractionated using gravity column chromatography. The fractions obtained were purified by microcolumn and preparative chromatography. To elucidate the structure chemistry of the molecules, nuclear magnetic resonance spectrometry was used and electrospray ionization mass spectrometry. As a result, two metabolites, a glycosylated flavonoid (nicotiflorin) and a phenolic derivative (1,3-di-O-transferuloylquinic), of the latter there is no precise pharmacological description.