Caracterización de metabolitos secundarios de Streptomyces sp. MJG08 y evaluación de su actividad antidermatofítica

Abstract

Abstract: Marine actinobacteria are potential producers of secondary metabolites with important biological activity. The objectives of this study were to characterize chemically and elucidate the structure of secondary metabolites of the Streptomyces strain sp. MJG08, as well as evaluate its antifungal activity. For fractionation of the extract crude oil, the column chromatography technique was used, using a mixture as mobile phase of chloroform-methanol in increasing polarity. The isolated compound was identified as 4- hydroxy-1-tetralone and has a molecular weight of 162.1 m/z. The chemical structure is assigned based on the 1H-NMR, 13C-NMR, HMQC, HMBC and COZY spectra, while that the mass was determined in the GC-MS. Using bioautography by agar overlay It was observed that the first 6 fractions show zones of inhibition against the fungus. Trichophyton rubrum, and by determining the MIC of these fractions, it was identified that 4-hydroxy-1-tetralone exerts antidermatophytic activity against T. rubrum at an MIC of 62.5 μg/mL (385.56 µM). Finally, it is concluded that the isolated compound has a moderate antifungal activity and likewise, the other fractions studied have bioactive compounds.