Comparación de tres métodos para el aislamiento de hongos micorrízicos arbusculares provenientes de cultivos trampa de plantaciones de café

Abstract

Abstract: Arbuscular mycorrhizal fungi (AMF) when associated with plants increase their access to water and nutrients from the soil, favoring their growth. For this reason, it is AMF represent an alternative to the use of conventional agrochemicals. The objective of this study was to compare between three isolation methods to evaluate which is more efficient and more successful. Monosporic spore culture methods were analyzed naked, encapsulation with alginate and cultivation from root fragments. Additionally, The average number of spores present and the most abundant morphotypes were determined. As a result of the test, it was determined that the most effective method for isolating HMA was cultivation from root fragments. It was also observed that, for every 100g of soil there was an average of 49.8 spores and that in general, the most abundant morphotypes They belonged to the genera Acaulospora and Glomus.