Determinación de la actividad biológica del aceite esencial de Melaleuca viridiflora

Abstract

Abstract: Melaleuca viridiflora is a species that belongs to the Myrtaceae family, it is a strong, robust and adaptable tree, its leaves are elongated and lanceolate of intense green color and small white flowers, this species is native to Australia and Asia. The essential oil of this species was obtained by steam distillation. The chemical composition was determined by gas chromatography equipped with a mass spectrophotometry and by gas chromatography coupled to flame ionization detector. The biological activity determined was antimicrobial, antifungal, antioxidant ant anticholinesterase. The antibacterial and antifungal activity was determined by the broth microdilution methods, the antioxidant activity was determined by two spectrophotometric methods ABTS and DPPH and the anticholinesterase activity was determined by the spectrophotometric method that determines the inhibiton of acetylcholinesterase. In the essential oil of Melaleuca viridiflora, 50 compounds were identified that represent 99.07% of its composition. The main componentes are cineole <1,8-> (35.66%), viridiflorol (14.2%), limonene (11.94%), terpineol <&#945;-> (7.89%), pinene <&#945;-> (7.42%). Melaleuca viridiflora essential oil did not show antimicrobial activity against the strains tested. For the antioxidant activity the oil showed moderate activity with activity SC50 1191.81 µg/mL for the ABTS method, 7657.47 µg/mL with DPPH method and for the anticholinesterase activity it presents moderate activity, with an IC50 65.98 µg/mL.