Aislar y caracterizar metabolitos secundarios del extracto en acetato de etilo de la especie Heliotropium rufipilum (Benth.) I.M.Johnst

Abstract

Abstract: In southern Ecuador, in the parish of El Tambo, several specimens of the species Heliotropium rufipilum (Benth.) I.M.Johnstm, belonging to the family Boraginaceae, were collected. In this sense, a phytochemical study was carried out in order to isolate and characterize the secondary metabolites of the plant. To achieve this purpose, the necessary extracts were obtained from the leaves, and the ethyl acetate extract was selected as the most suitable. Different procedures were carried out, such as thin layer chromatography and fractionation by column chromatography. These processes allowed the isolation of the main constituents, identified as 5-hydroxy-4',7-dimethoxyflavanone and Salvagenin, which constitute a mixture of two methoxylated flavonoids. The identification of these compounds was performed by NMR spectroscopic procedures (H1 and 13C), comparing the experimental data with previous studies. This analytical approach allowed confirming the presence and structure of the mentioned compounds in the studied plant.