Crioconservación y transporte de enterobacterales productoras de betalactamasas de espectro extendido y carbapenemasas

Abstract

Abstract: The glut microbiota is made up of microorganisms that perform important functions in the human organism, so that an alteration in their composition (morphological, physiological, genetic variability, etc.) leads to altered health conditions. Such is the case of Enterobacteriaceae that due to the inappropriate use of antibiotics have developed the production of extended spectrum beta-lactamases (EBLEE) and carbapenemases (EPC), where cryopreservation becomes an important tool for the study of these bacteria, however, bacteria lose the ability to produce these enzymes in prolonged storage at -80 °C. For this reason, it is intended to standardize cryopreservation and transport protocols that allow maintaining active EBLEE and EPC, for which cryopreserved cultures of bacteria identified and confirmed with EBLEE and EPC were used, which were subjected to stress with doses of antibiotics used in the confirmation of the production of these enzymes, were ultrafrozen at - 80 °C, performing periodic controls. It was observed that Enterobacteriaceae enhanced the production of EBLEE and EPC enzymes, however, the vitality of the bacteria was low due to multiple factors.