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http://dspace.utpl.edu.ec/handle/123456789/69533| Title: | Ability of 3 extraction methods (BCR, Tessier and protease K) to estimate bioavailable metals in sediments from Huelva estuary (Southwestern Spain) |
| Authors: | Rosado, Daniel Usero, José Morillo, José |
| Keywords: | Animals Endopeptidase K Environmental Monitoring Estuaries Geologic Sediments Metals, Heavy Polychaeta Spain Andalucia Huelva Estuary Huelva [Andalucia] Spain Arenicola marina Biochemistry Biomimetic processes Estuaries Positive ions Sediments Trace elements cadmium chromium copper iron lead manganese nickel proteinase K zinc heavy metal proteinase K BCR sequential extraction Bioavailable fraction Bioavailable metals Biomimetic approaches Huelva estuary Linear correlation coefficient Sequential extraction Trace metal bioavailability enzyme estuarine sediment extraction method trace metal Article bioavailability concentration (parameters) controlled study enzyme isolation estuary extraction and extracts intermethod comparison metal extraction sediment sequential extraction Spain animal chemistry environmental monitoring metabolism Polychaeta procedures Extraction |
| Issue Date: | 2016 |
| Publisher: | Elsevier Ltd |
| Abstract: | The bioavailable fraction of metals (Zn, Cu, Cd, Mn, Pb, Ni, Fe, and Cr) in sediments of the Huelva estuary and its littoral of influence has been estimated carrying out the most popular methods of sequential extraction (BCR and Tessier) and a biomimetic approach (protease K extraction). Results were compared to enrichment factors found in Arenicola marina. The linear correlation coefficients (R2) obtained between the fraction mobilized by the first step of the BCR sequential extraction, by the sum of the first and second steps of the Tessier sequential extraction, and by protease K, and enrichment factors in A. marina, are at their highest for protease K extraction (0.709), followed by BCR first step (0.507) and the sum of the first and second steps of Tessier (0.465). This observation suggests that protease K represents the bioavailable fraction more reliably than traditional methods (BCR and Tessier), which have a similar ability. © 2015 Elsevier Ltd. |
| URI: | https://dspace.utpl.edu.ec/bitstreams/2-s2.0-84956954026.pdf http://dspace.utpl.edu.ec/handle/123456789/69533 |
| ISSN: | 0025326X |
| Appears in Collections: | Artículos de revistas Científicas |
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