Aplicación de qNMR en la caracterización de Hernandulcin en la especie Phyla strigulosa

Abstract

Abstract: Ecuador counts with a high variety of plant species that offer the possibility to obtain metabolites that can contribute to the development of pharmaceutical products. The main purpose of this research is to quantify hernandulcin from the hexane extract of Phyla strigulosa. The compound was isolated using chromatographic techniques (CC and TLC) and analyzed by spectroscopic techniques (GC-MS, NMR). Consequently, an analytical method was developed in order to quantify the presence of hernandulcin in extracts of Phyla strigulosa using qNMR, a recent tecnique used in the quality control of vegetal extracts, in the field of metabolomics. In addition, the anticholinesterase activity of the hexanicnic extract was determined, whose inhibitory activity was weak with an IC 50 value of 89 µg/ml ± 1.16. The method was validated based on parameters of linearity, precision, accuracy, limit of detection, limit of quantification and recovery; obtaining results that are optimal to ensure its validation.