Desarrollo, validación y aplicación de un método analítico para la cuantificación de dehidroleucodina DhL a partir de hojas de Gynoxys verrucosa de la zona sur del Ecuador, usando cromatografía de gases acoplado a espectrometría de masas

Abstract

In the present research, an analytical method was designed, developed and validated for the quantification of dehydroleucodine in leaves of Gynoxys verrucosa collected in 4 different sectors of the south of Ecuador. This validated method was applied to 360 samples of the vegetable plants with the purpose of finding populations of G.verrucosa that produce more efficiently the metabolite. The parameters of linearity, accuracy, precision, recovery, limits of detection and quantification of the method were reached. The results were into the established parameters obtaining a good linearity (r^2 = 0.9978). The limits of detection (LOD) and quantification (LOQ) were 9.0515 ng/ul and 10.4256 ng/ul, respectively. The results of the recovery were between 81.06% - 116.61%, and the repeatability and reproducibility were less than 10% and 15% respectively. Thus, a simple, accurate, safe and reproducible GC/MS method was successfully developed and validated to analyze DhL in G.verrucosa extracts.