Detección de HPV en muestras parafinadas de las regiones anogenital y orofaríngea mediante PCR tiempo real

Abstract

The human papilloma virus is highly transmissible, producing the most common sexually transmitted infection in the world and inducing carcinogenesis in humans; it’s in the cervix where it has become more relevant being the main causative agent of cancer at this level, however, studies show that this virus also causes carcinoma in the oral, oropharyngeal and anogenital cavities, causing important health problems. The objective of this work was to determine the frequency and genotypes of HPV in samples of paraffined tissues with cytological alterations of oropharyngeal and anogenital regions; a total of 73 samples were processed from the pathological laboratory of the "Isidro Ayora" and "Solca Loja" hospitals for the years 2010, 2011, 2016 and 2017, to detect the virus by real-time PCR. It was found that 36.7% of valid samples presented HPV, with the female sex being the most affected (66.7%). Of the positive cases, 94.4% corresponded to the oropharyngeal region and 5.6% to the anogenital tract; the most frequent genotype was HPV 16 with 25.0%, followed by HPV 18 with 20.8%.