Aislamiento, identificación y actividad antimicrobiana de metabolitos secundarios a partir de las especies Lepechinia paniculata (Kunth) Epling y Piper lanceifolium Kunth

Abstract

The present investigation was based on the phytochemical study of the species Lepechinia paniculata and Piper lanceifolium, extracts of ethyl acetate and methanol were obtained from the leaves. The compounds were identified by NMR and GC-MS spectroscopic techniques and compared with reports in the literature. Three compounds of L. paniculata known as Ledol, Guaiol and Carnosol were isolated, and two compounds of P. lanceifolium, lanceaefolic acid methyl ester and cyclolanceaefolic acid methyl ester. Antimicrobial activity was determined by minimum inhibitory concentration (MIC). From L paniculata the ethyl acetate extract exhibited activity against Proteus vulgaris (62.5ug/ml), the methanol extract activity against Klebsiella pneumoniae (500ug/ml) and the compound Ledol activity against Klebsiella pneumoniae (125ug/ml) and Staphylococcus aureus (500ug/ml). The methanolic extract of P. lanceifolium was inactive against the strains used in the assay. The hypoglycemic activity of the extract and compounds of P. lanceifolium was also evaluated by the inhibitory capacity in α-glucosidase with an IC50 average of 114.73 μg / ml, 143.80 μg / ml, 147.94 μg / ml respectively.