Germinación in vitro de semillas de Encyclia aspera (Orchidaceae) una especie amenazada en Ecuador.

Abstract

Encyclia aspera is an epifita orchid found in Colombia, Peru and Ecuador; Very little is known about this species, which is synonymous with Epidendrum asperum. However, like the majority of orchids in the country, it is threatened by the massive extraction of wild specimens, as well as the destruction of their habitat. In vitro germination is an efficient method for the effective conservation of this species. In this work it was evaluated the asymbiotic germination of Encyclia aspera seeds using three cultivation means with different concentrations of naftalenacetico acid (ANA) and 6-benciladenina (BA) (2-0.5, 1-0.5 and 0.5-0.5 mg / L). The seeds were obtained from the UTPL`s germplasm bank (stored for six years), they were disinfected and seeded using the packed method in order to, after four weeks, evaluate the effect of different concentrations of phytohormones on the germination of Encyclia aspera. The results show that treatment 2 (1-0.5) was the most effective with a germination percentage of 52%.