Please use this identifier to cite or link to this item: http://dspace.utpl.edu.ec/handle/20.500.11962/23642
Title: Pruebas de crecimiento dual antagónico en medio agar sólido de hongos ascomycetes vs mycena citricolor
Authors: Cruz Sarmiento, Darío Javier
Albuja Narváez, Ricardo Miguel
Keywords: Ecuador.
Tesis digital.
Issue Date: 2018
Citation: Albuja Narváez, R. M. Cruz Sarmiento, D. J. (2018) Pruebas de crecimiento dual antagónico en medio agar sólido de hongos ascomycetes vs mycena citricolor [Tesis de Grado, Universidad Técnica Particular de Loja]. Repositorio Institucional. https://dspace.utpl.edu.ec/handle/20.500.11962/23642
Abstract: Abstract:Coffee is an agricultural product of economic importance for farmers in Ecuador, However, it`s cropping affected by different factors being pathogenic fungi such as the Basidiomycete pathogenic fungi Mycena citricolor, very harmful for coffee crops. The present study is seeking to find antagonistic fungi within Ascomycetes (Trichoderma spp.) as controler or competitive againts pathogenic species testing antagonism on agar medium and fungal extracts. All the evaluated strains were molecularly characterized, where four different species (Trichoderma asperellum, Trichoderma harzianum, Trichoderma sp1 and Trichoderma sp2) were identified. The percentages of inhibition were statistically evaluated acoording to antagonistic tests between controller/pathogen, an also including radial growth of controller and pathogenic fungus. The results shows that three of the four species evaluated as controllers were effective with inhibition values greater than 40% with significant differences (p-value <0.05) among some treatments. These species are potential biocontrollers of the pathogen M. citricolor.Coffee is an agricultural product of economic importance for farmers in Ecuador, However, it`s cropping affected by different factors being pathogenic fungi such as the Basidiomycete pathogenic fungi Mycena citricolor, very harmful for coffee crops. The present study is seeking to find antagonistic fungi within Ascomycetes (Trichoderma spp.) as controler or competitive againts pathogenic species testing antagonism on agar medium and fungal extracts. All the evaluated strains were molecularly characterized, where four different species (Trichoderma asperellum, Trichoderma harzianum, Trichoderma sp1 and Trichoderma sp2) were identified. The percentages of inhibition were statistically evaluated acoording to antagonistic tests between controller/pathogen, an also including radial growth of controller and pathogenic fungus. The results shows that three of the four species evaluated as controllers were effective with inhibition values greater than 40% with significant differences (p-value <0.05) among some treatments. These species are potential biocontrollers of the pathogen M. citricolor.
Description: Resumen: El café es un producto agrícola de importancia económica para el Ecuador, el cual se ve afectado por múltiples factores, siendo el ataque de hongos patógenos como por ejemplo el Basidiomicete Mycena citricolor, el cual puede ser muy perjudicial para este cultivo. Este estudio busca encontrar hongos antagónicos como los Ascomycetes donde existen especies controladoras o competidoras de plagas como por ejemplo Trichoderma spp, mediante pruebas de antagonismo en medio agar y a través de extractos fúngicos. Las cepas evaluadas fueron caracterizadas molecularmente, determinando cuatro especies diferentes T. asperellum, T. harzianum, Trichoderma sp1 y Trichoderma sp2. Se evaluó estadísticamente los porcentajes de inhibición en pruebas antagónicas controlador/patógeno, además de crecimiento radial de micelio controlador y patógeno. Determinando que tres de las cuatro especies de Trichoderma evaluadas fueron efectivas con valores de inhibición superiores al 40% y significativamente diferentes (p-valor <0.05) entre algunos tratamientos. Estas especies de Trichoderma son potenciales biocontroladores del patógeno M. citricolor.
URI: https://bibliotecautpl.utpl.edu.ec/cgi-bin/abnetclwo?ACC=DOSEARCH&xsqf99=120419.TITN.
Appears in Collections:Biólogo



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