Determinación de las propiedades físicas, caracterización química y evaluación de la actividad biológica del aceite esencial de Cúrcuma (Cúrcuma Longa).

Abstract

Abstract:Cúrcuma longa is a species native to the humid tropical and subtropical regions of Asia, the essential oil was extracted from its rhizomes by hydrodistillation. The chemical composition of the essential oil was determined by gas chromatography coupled to mass spectrometry CG/MS and flame ionisation detector CG/FID. The biological activity was evaluated by the microdilution method in broth and the antioxidant activity by two spectrophotometric methods ABTS and DPPH. The physical properties of the oil were also determined. Nineteen compounds were identified with an identification percentage of 97,85% in the non-polar column (DB5-MS) and 97,08% in the polar column (HP-Innowax). The majority compounds were: ar-Turmerone (40,74%), -Turmerone (15,07%), -Turmerone (11,62%)-Phellandrene (9,05%) and 1,8-Cineole (4,30%). The density of essential oil was 0,9193 g/cm3, the refractive index was 1,5006 and the optical activity was -40,792°, the essential oil showed no biological activity against the bacterial or fungal strains. A higher antioxidant potential was determined by the ABTS method with an average SC50 less than 0,5 mg/mL.