Efecto quimiopreventivo de las horchatas expendidas en la ciudad de Loja

Abstract

Abstract:Horchata is a beverage prepared from medicinal plants, that have several biological properties reported, of which the one we are interested in, is its possible chemopreventive activity. This, because lately preventive strategies have taken space in the investigation field. According to previous studies horchatas possess a citotoxic effect on the astrocytoma cell line (D-384), besides having an antigenotoxic effect on the Chinese Hamster Ovary cell line (CHO-K1). Our purposes were determining the genotoxic and antigenotoxic capability of 4 horchatas mixes (H1, H2, H7, H9). Initially we evaluate the cytotoxic activity of various doses of horchatas by the double staining assay FDA / EtBr, obtaining subtoxic doses, propitious to later determine the genotoxic and antigenotoxic effect through the comet assay. Our results showed through the tail length parameter that horchatas H1, H7 and H9 at a 1000 g/mL concentration produced genotoxicity, however, H2 does not produce such effect. On the other side, possess antigenotoxic effect on human leukocytes, as desmutagenic agents H2, H7 and H9, or as a biomutagenic agent H1.