Análisis del efecto clonogénico y ciclo celular del extracto metanólico de Grias neuberthii sobre líneas celulares tumorales humanas

Abstract

Abstract:Cancer is the process in which cells replicate in an uncontrolled manner and get spread by metastasis. To counteract cancer, millions of people in the world turn to traditional medicine. In Ecuador, we can find the species Grias neuberthii Macbr, whose medicinal use is considered in the southern region of the country. The present work is developed with the purpose of identifying the cytotoxic and cytostatic capacity that the methanolic extract of Grias neuberthii induces on tumor cells RKO with normal p53 and SW613-B3 with p53 mutated through the clonogenic assay and the analysis of the cell cycle by cytometry of flow. The results show that the extract does not stop the cell cycle in any of the two cell lines; whereas, in the clonogenic assay when using the CI50, the RKO cell line reduces its ability to form colonies, whereas SW613-B3 has been shown to have greater resistance to treatments with antiproliferative extracts by virtue of the p53 state.