Validación de métodos analíticos para la determinación de Escherichia coli, Salmonella y Cryptosporidium en las matrices de: agua natural, de consumo humano y residual

Abstract

Abstract:In order to have reliable results in laboratories, it is essential to have validated methodologies that make it possible to assert that the method used is appropriate for the purpose proposed. Therefore, it is important that the methodologies used in the different tests and in the different matrices are validated. The main objective of this work was the validation of analytical methods to determine Escherichia coli, Salmonella and Cryptosporidium in the matrices of: natural water, human consumption and residual. The validation was performed using standardized methods using certified reference material (pure strains). The membrane filtration method was used to determine Escherichia coli, the 3M Petrifilm Salmonella Express System (SALX) system was used for Salmonella, and the Zielh Nelseen stain was applied for Cryptosporidium. 15 replicates were made for each matrix with the respective recoveries using the pure strains. The validation parameters to be determined were established and with the information obtained the statistics were determined that allow us to establish if the method is suitable for the proposed application. Once the validation of the method was confirmed, the protocols of each method and the respective validation summaries were elaborated.