Extracción, aislamiento, caracterización de metabolitos secundarios y evaluación de la actividad biológica de la especie Colicodendron scabridum (Kunth) Seem

Abstract

Abstract:In the present phytochemical study of the species Colicodendron scabridum Kuth Seem, known as dog sapote and collected in the canton Catamayo of the province of Loja, seven compounds were identified: four terpenes named Lupeol, Lutein, a mixture of β-sitosterol and Stigmasterol; Two pyrrolidinic alkaloids known as stachyhydrin and betonicin and a glycosylated flavonol called narcissoside. For the elucidation of the molecules, spectroscopic techniques of NMR, HPLC-MS, GC-MS were used and corroborated with the data from the literature. In addition, the antimicrobial activity was carried out using CMI against bacteria such as Staphylococcus aureus or Escherichia coli and fungi such as Candida albicans, which is inactive for extracts of hexane, ethyl acetate and methanol. An antioxidant activity was performed for the DPPH method, an AAI index of 0.14, 0.02 and 0.06 for the methanol, ethyl acetate and hexane extracts.