Validación de un método analítico por Cromatografía Líquida de Alta Resolución acoplada a Espectrometría de Masas (HPLC-MS) para la determinación y cuantificación de Hernandulcin obtenido de la especie Phyla strigulosa

Abstract

Abstract:The present investigation aims to validate and quantify Hernandulcin from the hexane extract Phyla strigulosa, using an analytical method in the equipment of High Performance Liquid Chromatography coupled to mass spectrometry (HPLC-MS). To carry out the validation, five concentrations of the extract were read; where the statistical analyzes were carried out, reaching satisfactory results according to the established ranges, obtaining a good linearity (R ^ 2 = 0,9996), as well as the limit of detection and quantification (23,3 ng / uL and 90,1 ng / uL respectively). In addition, a correct recovery was achieved that was between 80% and 120%; In terms of repeatability and reproducibility, the results were lower than 10% and 15% respectively. With respect to the quantification of Hernandulcin, it was possible to obtain 12.31%. Therefore, the method is sufficiently sensitive, accurate and reproducible in HPLC-MS for the extract rich in Hernandulcin, which is suitable for use in a variety of alternative applications: food, pharmaceutical and oral hygiene.