Determinación de la composición química, análisis enantiomérico y actividad biológica del aceite esencial de las partes aéreas de Siparuna muricata en diferentes poblaciones de la provincia de Loja.

Abstract

Abstract: In the following research the study of the essential oil of the species Siparuna muricata known as "limoncillo" was performed, extracted through steam distillation, in different localities of the province of Loja, in which was characterized the chemical composition of the essential oil by means of GC-MS and GC-FID, a total of 44 compounds were identified representing the 84,59% 92,50%, 91,83%, and 87,20%, corresponding to the areas of Chuquiribamba, Yangana, Celica and Colaisaca, respectively. It was also determined the enantiomeric distribution by GC-MS in the capillary column of stationary phase MEGA-DEX-DET-BETA, allowing the enantiomeric separation of -Pinene, Limonene, -Elemene-Bourbonene, cis- Cadina-1 (6), 4-diene and Atractylone. We evaluated the biological activity of the oil: antifungal and antibacterial without presenting inhibitory, and antioxidant activity in spectrophotometric tests, in DPPH did not present reduction significant results, and in ABTS we visualized significant results just in the essential oil from Chuquiribamba, with SC50= 14,88 g/mL. Additionally, different physical properties of the essential oil were assessed, as yield 0,135%, density 0, 913g/ml, refractive index 1,504 ntD and optical activity.