Evaluación in vitro del efecto acaricida de eucaliptol y alfa pineno sobre la garrapata del ganado Rhipicephalus (Boophilus) microplus

Abstract

Abstract:In the present study, the acaricidal efficacy of eucalyptol and α-pinene was evaluated on Rhipicephalus (Boophilus) microplus. Ticks were collected in Guachanamá parish in Loja province and incubated for oviposition. The 19-day larvae were subjected to the larval package test, with concentrations of 2.5, 5, 10, 15 and 20 mg / ml of eucalyptol and α-pinene. The compounds were diluted in a solution of ethanol: 50:50 water. For each concentration, five repetitions were conducted. A 50:50 ethanol / water and water mixture were used as negative controls. At the highest concentration (20mg / mL), eucalyptol caused a mortality value of ˂35%; mortality value with α-pinene was ˂28%. The correlation values between the concentration of the compounds and the mortality were strong and positive (0.976 and 0.956 for eucalyptol and α-pinene, respectively). The compounds eucalyptol and α-pinene have low acaricidal efficacy; however, these compounds should be evaluated in different populations of ticks and in adult females to evaluate their effect on reproductive parameters such as oviposition and hatching of larvae.