Validación de un método analítico por Cromatografía Líquida de Alta Resolución acoplada a Espectrometría de Masas (HPLC-MS) para la determinación y cuantificación de pioglitazona y donepezilo en una microemulsión

Abstract

Abstract:The present research project of investigation have aim to perform the validation and quantification of pioglitazone and donepezil in a microemulsion, using an analytical method in the team of High Resolution Liquid Chromatography coupled to Mass Espectometry (HPLC-MS). The validation method was performed using 5 concentration of each the compound; where it was obtained in the linearity parameter a R2 (0,9987) pioglitazone and R2 (0,9999) for donepezil; in relation with the detection limit and quantification, it was obtained for pgz (0,0804 µg / mL-0,0835 µg / mL) and DNP (0,0867 µg / mL-0,0944 µg / mL); in the regards to repetibility and reproducibility the percentages of the coefficient of the variation where less than 10 and 15%. The accuracy was determined from the percentages of recuperation of each compound where each one complies with the ranges between 80 to 120%, it concludes that each result of the parameters of validation accomplish with the stipulated according to the international standards of analytical validation; where is certify that is reliable and reproducible for subsequent studies.