Aislamiento y caracterización molecular de cepas de bacterias solubilizadoras del fósforo.

Abstract

Abstract:Microorganisms play important roles, such as uptake and transfer of nutrients to soil-plants. Phosphorus is the second most important macronutrient for plant nutrition after nitrogen and its deficiency can limit its growth as it is necessary in various cellular processes such as DNA and ATP synthesis. Phosphorus in the soil is found organically and inorganically; an average soil contains approximately 0.5% phosphorus and only 0.1% available for plants, so farmers to meet phosphorus needs in their crops make organic amendments with phosphoric rock. In this research, bacteria from the rhizosphere taken at the UTPL Agricultural Station, located in the Cajanuma - Loja sector, were isolated. Bacterial isolates were characterized morphologically and molecularly, evaluating their phosphorus solubilizing capacity. 24 morphotypes were identified from the morphological data collected. The 16S ADNr region analysis determined the genera. Of the strains identified, 33.33% were able to solubilize phosphorus, highlighting the genera Bacillus, Pseudomonas and Arthrobacter.