Amplificación de la región LCR de HPV 18 de muestras citológicas de la ciudad de Loja.

Abstract

Abstract:Human papillomavirus (HPV) is considered one of the most common sexually transmitted diseases in young women, the HPV strain 18 is the second most common genotype associated with cervical adenocarcinoma. This condition is a public health problem in Latin America, because of this, this research topic has been raised, with the objective of determining the variants of HPV 18 circulating in the city of Loja, through the amplification, sequencing and phylogenetic analysis of the LCR region of the virus. Of the amplified samples, a 16% complementarity of the virus to its genome was obtained by comparing them with the NCBI database, obtaining at the phylogenetic level a taxonomic association towards lineage A of HPV 18, the low complementarity of the virus is attributed to conservation of DNA in biobanks, so it is recommended to take this research as a reference for the development of future analyzes.