Actividad genotóxica mediante el ensayo cometa de 16 derivados de tipo bis (espiro-2,4-dihidro-3H-pirazol-3-ona) ciclopropano

Abstract

Abstract: The search for new therapeutic agents has been directed towards the synthesis of new chemical derivatives based on known structures, such as pyrazole derivatives, which exhibit a wide variety of biological activities. As part of the safety profile of the new chemical entities, it is necessary to assess the toxicological risks, including genotoxicity, that is, their ability to cause DNA damage. For this, the current research work has focused on evaluating the genotoxic activity of 16 bis (spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropane-type derivatives, synthesized from pyrazoles; against a biological model of healthy cells, the CHO-K1 cell line, and by the comet assay, a well-known genotoxicity biomarker. The results allowed to compare the parameters of tail length, moment and tail intensity, as well as GDI, demonstrating that from the 16 derivatives, the 4d derivative showed significant differences compared to untreated cells in the three parameters, and whose DNA damage can be assesed to the presence and position of the NO2 functional group within the molecule.