Aislamiento e identificación de metabolitos secundarios de la especie Myrcianthes fragrans (Sw.) McVaugh

Abstract

Abstract: In the present investigation, the phytochemical study of secondary metabolites was made from the leaves of the species Myrcianthes fragrans (Sw.) McVaugh, known as bayberry. For the purification of the total methanol extract and the extracts: chloroform, precipitate, lyophilisate and aqueous, obtained by static maceration, liquid-liquid fractionation and rota-evaporation, chromatographic techniques such as thin layer chromatography and column chromatography were used to fractionate the extract. The precipitate was made using the Reveleris® PREP Purification System from BUCHI. Using NMR spectroscopic techniques and data reported in the literature, a triterpenic compound known as ursolic acid obtained from the fractionation of the methanolic extract and a flavonoid compound known as quercetin obtained from the fractionation of the precipitated extract were identified. A sample of the precipitated extract was sent to the Montpellier Cancer Institute in France where the biological activity was evaluated, the precipitated extract showed a high inhibitory activity on the enzyme Butyrylcholinesterase (BuChE) with an IC50 of 18.2 ± 4.5 µg / ml, while the The extract did not show inhibitory activity against the enzyme Acetylcholinesterase (AChE)