Método para determinar el factor de protección solar (FPS) in vitro

Abstract

Abstract: The Ecuadorian territory receives levels of ultraviolet radiation (UV) above the established ones these levels reach values up to 14-16UV WHO has set the maximum tolerable for human exposure to 11UV. The purpose of this study is to determine the sun protection factor of a photoprotective cream and in turn make a comparison between photoprotective creams of different brands with different Sun Protection Factor (SPF). We have used an in vitro technique, implementing an organic dye being curcumin the most viable for ease of extraction, the organic dye allows us to directly observe the degradation produced by UV radiation. Glass substrates are used in which we place 100 µl of solution with dye in a centrifuge system at 2500 rpm, obtaining a thin film of dye without solvent; they are introduced into a UV transparent test cell covered by a thin film of the photoprotective cream under study. This cell is placed in an ultraviolet radiation chamber for a time ranging from 0-150 min. The number of manufactured substrates is subjected to image analysis to obtain a trend line with respect to the degradation time to the exposure to ultraviolet radiation, thereby determining the degradation curve photoprotective creams mark.