Determinación de mutaciones puntuales del gen BRAF, asociado a cáncer de tiroides a partir de muestras parafinadas provenientes de SOLCA, Loja

Abstract

Abstract: Thyroid cancer is one of the most frequently occurring endocrine neoplasms, with women being the most affected. The type of thyroid cancer is classified as one of: papillary, follicular, medullary or anaplastic carcinoma. BRAF gene mutations are the main causes of this cancer. The method for DNA extraction was standardized with 12 paraffin tissue samples from patients diagnosed with thyroid cancer from the SOLCA Loja hospital, and clinical data from 25 patients with benign and malignant thyroid nodules were collected. As a result of the standardization, a procedures manual was prepared and the results of the clinical data collection show that most of the patients come from the city of Loja, the most frequent type of cancer is papillary carcinoma, with the most susceptible patients being married women aged between 51 and 60 years old. Hypothyroidism, hypertension, obesity, and diabetes were the most frequently occurring underlying diseases related to thyroid cancer, and the most common surgical intervention was thyroidectomy.